Human Superoxide Dismutase 2, Mitochondrial (SOD2) ELISA Kit

Mitochondrial antioxidant research relies on precise quantification of superoxide-scavenging enzymes, and this quantitative ELISA Kit from Assay Genie targets SOD2 (MnSOD), the manganese-dependent isoform that localizes to the mitochondrial matrix. SOD2 catalyzes the conversion of superoxide radicals into hydrogen peroxide and molecular oxygen, acting as a primary defense against oxidative damage generated through aerobic respiration.

Researchers studying neurodegeneration, cardiovascular pathology, tumor biology, chronic inflammation, and cellular senescence use SOD2 expression as an indicator of mitochondrial redox balance. The kit accommodates diverse human-derived specimens, including tissue homogenates, cell lysates, and conditioned media from cultured cells, supporting both in vitro and in vivo experimental designs. Each kit contains a pre-coated microplate and the reagents needed to generate a standard curve for interpolating unknown sample concentrations against a calibrator of known SOD2 content.

SOD2 is encoded in the nucleus and imported into mitochondria via a cleavable N-terminal presequence. Its transcription is upregulated by oxidative challenge through NF-κB, AP-1, and FoxO signaling pathways, making it a dynamic biomarker of antioxidant capacity under pharmacological, environmental, or genetic perturbation. The functional enzyme exists as a homotetramer of approximately 25 kDa subunits and requires manganese as a catalytic cofactor at its active site, distinguishing it biochemically from the cytosolic Cu/Zn SOD1 isoform.