Human Sulfite oxidase, mitochondrial (SUOX) ELISA Kit

Assay Genie offers a Sandwich-format ELISA Kit for the quantitative detection of human sulfite oxidase, mitochondrial (SUOX) in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Intended for research use only, the kit employs a colorimetric ELISA detection method with reactivity specific to human samples. The target analyte is a homodimeric enzyme localised to the mitochondrial intermembrane space (EC 1.8.3.1) and is involved in the metabolism of sulfur-containing amino acids and the detoxification of sulfite.

Reported performance characteristics include a detection range of 15.6-1000 pg/mL, a sensitivity of 7.8 pg/mL, an intra-assay CV of 7.9% and an inter-assay CV of 11.1%. Specificity is established for natural and recombinant human SUOX (UniProt P51687). Linearity has been characterised across serum, EDTA plasma and heparin plasma dilutions from 1:2 to 1:16, with recovery averaging 88% (range 82-94%) for serum and 90% (range 84-96%) for plasma.

The kit is supplied with a dismantlable ELISA microplate, lyophilised standard, sample and assay diluents, detection reagents A and B, wash buffer, substrate solution, stop solution and plate sealer, each with specified storage conditions. The assay protocol uses a 450 nm microplate reader with 37°C incubation steps.