Human Sulfiredoxin-1 (SRXN1) ELISA Kit

Designed for the precise quantitative measurement of Sulfiredoxin-1 (SRXN1) in human samples, this ELISA Kit from Assay Genie supports research into oxidative stress and redox biology. SRXN1 contributes to oxidative stress resistance by reducing cysteine-sulfinic acid formed under exposure to oxidants in peroxiredoxins PRDX1, PRDX2, PRDX3 and PRDX4, but does not act on PRDX5 or PRDX6, catalysing the reduction via a multi-step process as both a specific phosphotransferase and thioltransferase. Dysregulation of SRXN1 has been implicated in cancer, neurodegenerative disorders and metabolic syndromes, making it a valuable biomarker candidate for understanding disease mechanisms and exploring therapeutic interventions.

The kit is validated for serum, plasma, tissue homogenates, cell culture supernatants and other biological fluids, and detects both natural and recombinant human Sulfiredoxin-1. Performance characteristics include a detection range of 0.625-40ng/mL and a sensitivity of 0.338ng/mL. Intra-assay CV is 7.5% and inter-assay CV is 9.7%. Linearity has been demonstrated in serum (105-114%, 100-110%, 87-97%, 100-110% at 1:2, 1:4, 1:8 and 1:16 dilutions respectively), EDTA plasma (85-94%, 94-105%, 116-126%, 104-114%) and heparin plasma (90-100%, 102-111%, 85-94%, 100-111%). Recovery averages 103% (range 97-109%) for serum and 105% (range 99-111%) for plasma. The target protein is localised to the cytoplasm (UniProt Q9BYN0) and carries the synonyms C20orf139, SRX and SRX1. For research use only.