Human Sucrase Isomaltase (SI) ELISA Kit

The Human Sucrase Isomaltase (SI) ELISA Kit from Assay Genie enables quantitative measurement of sucrase-isomaltase, a key intestinal brush-border hydrolase responsible for hydrolyzing sucrose, maltose, and isomaltose during carbohydrate digestion. Researchers studying congenital sucrase-isomaltase deficiency (CSID), carbohydrate malabsorption syndromes, inflammatory bowel disease, and intestinal mucosal integrity apply this assay to evaluate enzyme levels in tissue homogenates. The kit provides reagents for 96 individual determinations arranged across a microplate strip frame, with a sandwich capture-and-detection format. Each well accommodates a small sample volume of 50 µL, and the included standards support construction of a calibration curve for interpolation of unknown concentrations. Reagents are supplied ready-to-use in sealed vials and should be refrigerated at 4 °C prior to first use to preserve antibody activity; avoid repeated freeze-thaw cycles of the lyophilized standards. Detection relies on an enzymatic substrate producing a measurable absorbance change, with results analyzed against the standard curve to derive ng/mL values. Intended for in vitro research use only, the kit supports studies in gastroenterology, pediatric digestion disorders, glycobiology, and brush-border enzyme biology.