Human Succinate Dehydrogenase Complex Subunit B (SDHB) ELISA Kit

This ELISA from Assay Genie quantifies human SDHB, the iron-sulfur subunit of mitochondrial succinate dehydrogenase (complex II), enabling research into mitochondrial bioenergetics, the citric acid cycle, and electron transport chain function. SDHB protein levels are actively investigated in hereditary paraganglioma-pheochromocytoma (PGL/PCC) syndrome studies, Carney-Stratakis dyad, succinate-driven HIF-1alpha stabilization, SDH-deficient gastrointestinal stromal tumors (GIST), and renal cell carcinoma metabolism, where loss of expression is diagnostic of complex II disruption.

Applied to tissue homogenates, cell lysates, and protein extracts from human-derived research specimens, the kit is supplied as a research-use-only microplate package with pre-coated wells and accommodates a 100 uL sample volume per well. Investigators commonly pair SDHB quantitation with western blot or immunohistochemistry profiling of SDHA, SDHC, and SDHD to evaluate complex II subunit integrity in patient-derived tumor samples. Reagents should be kept refrigerated, with the standard and detection antibody protected from repeated freeze-thaw cycles to preserve activity across multiple runs.