Human STXBP4 ELISA Kit (HUFI06268)

Syntaxin Binding Protein 4 (STXBP4), a cytoplasmic regulator of SNARE-mediated vesicle fusion, is the target analyte quantified by this Assay Genie ELISA Kit. The protein has been investigated in connection with insulin granule exocytosis, membrane trafficking, and glucose homeostasis, and the kit supports measurement of endogenous human STXBP4 in experimental samples used in cell-based and biochemical workflows.

The assay follows a microplate-based protocol in which capture antibodies are pre-immobilized on the plate, and bound analyte is revealed through an enzyme-conjugated detection reagent developed with a chromogenic substrate. Each kit supplies pre-coated wells, detection antibody, reference standards, wash buffer concentrate, substrate solution, and stop reagent, providing complete materials for a single plate run. Researchers typically load unknown samples alongside a standard curve to interpolate STXBP4 concentrations from the resulting optical readings.

STXBP4, also known as Synip, binds syntaxin-4 and has been linked to regulation of GLUT4 vesicle trafficking in adipocytes and skeletal muscle cells. This places the protein at the center of metabolic disease research, including investigations into type 2 diabetes, insulin resistance, and obesity-related signaling pathways. Cell lysates and tissue homogenates prepared in non-denaturing lysis buffer are commonly used as input material, and proper handling — keeping lysates on ice and limiting freeze-thaw cycles — helps preserve epitope integrity for accurate quantification. For research use only.