Human sTNFRSF12A (Soluble Tumor Necrosis Factor Receptor Superfamily, Member 12A) ELISA Kit (HUES03357)

The Assay Genie sandwich ELISA operates on a 96-well microplate pre-coated with a capture antibody specific to the extracellular domain of human TNFRSF12A. Soluble Fn14 present in serum, plasma, urine, or cell culture supernatant is captured during incubation, then detected with a biotinylated detection antibody followed by streptavidin-HRP conjugate. The enzymatic reaction with TMB substrate produces a measurable color change proportional to sTNFRSF12A concentration, with absorbance typically read at 450 nm within 15 minutes of stop solution addition. Each kit supplies a pre-coated plate, lyophilized standards, detection antibody, streptavidin-HRP, TMB substrate, wash buffer concentrate, sample diluent, and stop solution, sufficient for 96 determinations run in duplicate. The standard curve range generally spans 15.6 to 1000 pg/mL with intra-assay precision under 10% CV, supporting reproducible quantification in cardiovascular biomarker studies, chronic kidney disease research, and tumor microenvironment investigations. Reagents should be stored at 4 degrees C, with the standard stock aliquoted and held at -20 degrees C or lower to limit repeated freeze-thaw exposure, and the working streptavidin-HRP solution should be protected from light. For research use only, not intended for diagnostic procedures.