Human STK (serine/threonine protein kinase) ELISA Kit (HUFI04732)

This Assay Genie kit enables quantitative measurement of serine/threonine kinase levels in human samples, supporting research into kinase-mediated signal transduction, phosphorylation cascades, and intracellular cell-signaling pathways. The protocol uses 100 uL of sample per well, allowing reproducible detection of low-abundance kinase expression across multiple clinical and experimental matrices.

Each kit ships with a pre-coated microplate, biotinylated detection antibody, streptavidin-HRP conjugate, recombinant kinase standards, wash buffer, substrate solution, and stop reagent sufficient for duplicate determinations. Reconstituted components should be kept refrigerated between runs and protected from light, with working dilutions prepared fresh before each plate.

Researchers commonly apply this ELISA in oncology target validation, biomarker discovery, and drug-development workflows where kinase activity or expression is being profiled in patient cohorts and experimental models.