Human STC2/Stanniocalcin-2 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the quantitative measurement of STC2 (Stanniocalcin-2) levels in human samples using a double-antibody colorimetric detection format. The kit is reactive with human analytes and supports research in cell biology, where STC2 has been studied for its role in cell growth, differentiation, and inflammation regulation. Dysregulation of STC2 has been associated with conditions such as cancer, diabetes, and cardiovascular disorders, making the protein a useful target for biomarker investigation.

Validated sample matrices include serum, plasma, cell culture supernatant, cell or tissue lysates, and other liquid samples. Reported assay performance includes a sensitivity of 18.75 pg/mL and a working range of 31.25-2000 pg/mL. Recovery performance across matrices has been reported for serum (85-104%, average 96%), EDTA plasma (85-98%, average 91%), and heparin plasma (85-104%, average 98%), with linearity data across serial dilutions of these same matrices. Reagents are stored at 2-8°C for 12 months per the manufacturer's protocol.

The procedure involves a primary incubation at 37°C for 90 minutes, followed by sequential binding of a biotin-labelled detection antibody and HRP-Streptavidin, with TMB substrate development and absorbance reading at 450 nm. Annotated synonyms for STC2 include STC-2, Stanniocalcin-related protein, STC-related protein, and STCRP, with the UniProt reference O76061.