Human STAT4 ELISA Kit

Assay Genie's Human STAT4 ELISA Kit is a sandwich-format, double-antibody immunoassay designed for the accurate quantification of Signal Transducer and Activator of Transcription 4 (STAT4) in human samples. STAT4 is a key transcription factor involved in regulating immune responses, particularly in the development of T helper 1 cells and the production of pro-inflammatory cytokines, and its dysregulation has been linked to various autoimmune diseases, making it a relevant biomarker in immunological research.

The kit is reactive with human samples and has been validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. It delivers a quoted sensitivity of 0.188 ng/mL and a measuring range of 0.313–20 ng/mL. Spiked recovery averages were 96% for serum (range 90–105%), 96% for EDTA plasma (range 88–103%), and 94% for heparin plasma (range 85–105%). Dilutional linearity in serum, EDTA plasma, and heparin plasma fell broadly between 82% and 105% across 1:2, 1:4, and 1:8 dilutions. Storage is at 2–8°C for 12 months.

The assay workflow uses a pre-coated plate with standards, samples, and controls incubated at 37°C for 90 minutes, followed by a biotinylated detection antibody (37°C, 60 minutes), HRP-streptavidin binding (37°C, 30 minutes), TMB development in the dark for 10–20 minutes, and a stop reaction read at 450 nm. Provided reagents include a dismountable ELISA microplate, lyophilized standard, biotin-labeled antibody concentrate, HRP-streptavidin conjugate, TMB substrate, sample, antibody, and SABC dilution buffers, stop solution, 25X wash buffer, plate sealer, and a technical manual. The kit is associated with the Epigenetics and Nuclear Signaling research area, with the STAT4 protein also referenced by the synonym SLEB11.