Human SSX2IP ELISA Kit (HUFI06332)

SSX2IP, the Synovial Sarcoma X Breakpoint 2 Interacting Protein, is a cytoplasmic scaffolding component that anchors the nectin-afadin complex at adherens junctions, links those junctions to the actin cytoskeleton, and contributes to Hippo pathway signaling and mitotic spindle orientation. Researchers investigating epithelial polarity, cell-cell adhesion, and tumor invasion use SSX2IP as a marker of junctional integrity, particularly in hepatocellular carcinoma and lung adenocarcinoma studies, where loss of SSX2IP correlates with epithelial-mesenchymal transition.

The Assay Genie Human SSX2IP ELISA Kit delivers a quantitative, plate-based readout for SSX2IP across peripheral blood-derived specimens, including serum fractions and matched plasma preparations, plus cell culture supernatants and tissue lysates from human donors. Each kit includes pre-coated microplate wells, lyophilized standards, detection antibody, concentrated buffers, wash solution, and substrate sufficient for 96 individual determinations. Reconstituted standards and working detection antibody should be aliquoted to limit freeze-thaw cycles, and substrate reagents must be protected from light during use.

Intended for research use only, the kit supports investigators working on cancer cell biology, signal transduction, and biomarker discovery. Assay Genie supplies a standard operating manual with each lot describing recommended sample volume, the calibration curve range, and analytical sensitivity so that users can adapt the protocol to their particular workflow. Standard laboratory microplate readers capable of measuring absorbance in the visible range are sufficient to capture the final readout.