Human SPTAN1 (Alpha-Fodrin) CLIA Kit (HUES00873)

From Assay Genie, this chemiluminescence immunoassay kit is designed for the in vitro quantitative determination of Human SPTAN1 (Alpha-Fodrin) concentrations in serum, plasma and other biological fluids. SPTAN1 is a cytoskeletal protein involved in cell structure and function, with research relevance to neurodegenerative disorders and autoimmune diseases, supporting investigation into those conditions.

The kit uses the Sandwich-CLIA principle, with a microplate pre-coated with an antibody specific to Human SPTAN1. Standards or samples are combined with a biotinylated detection antibody and an Avidin-Horseradish Peroxidase (HRP) conjugate, and the relative light unit (RLU) value is read on a chemiluminescence immunoassay analyzer. The assay has a stated sensitivity of 18.75 pg/mL and a detection range of 31.25-2000 pg/mL, with a total assay time of 4.5h. The required sample volume is 100 μL.

Specificity is reported as no significant cross-reactivity or interference between Human SPTAN1 and analogues. Reproducibility is stated as both intra-CV and inter-CV < 15%.