Human SPRY4 ELISA Kit (HUFI06350)

The SPRY4 protein, a member of the Sprouty family of negative feedback regulators, plays a pivotal role in modulating receptor tyrosine kinase signaling through the MAPK/ERK pathway. Assay Genie offers a quantitative immunoassay kit formatted for 96 Assays, intended for in vitro research measuring SPRY4 concentrations in human samples and supporting work in cell signaling, oncology, and developmental biology.

Built around specific antibody pairs that capture and detect SPRY4, the assay supports investigations into fibroblast growth factor (FGF)-mediated pathways, angiogenesis, and tumor cell proliferation. Researchers studying melanoma, prostate, or breast cancer biology frequently employ SPRY4 quantification to explore its role as a tumor suppressor and downstream effector of MAPK signaling. The kit arrives with all necessary reagents, plates, and buffers packed for the workflow.

Compatible specimens include liquid human samples handled under standard collection protocols. To preserve SPRY4 integrity, aliquot samples upon receipt and minimize freeze-thaw cycles. Reconstituted reagents should be refrigerated and stored according to the supplied insert.

Beyond oncology, SPRY4 measurement supports research into pulmonary fibrosis, where dysregulated FGF signaling contributes to disease progression, and reproductive biology where Sprouty proteins regulate gonadal development. The protocol accommodates standard laboratory workflows and ELISA plate readers, making it a practical option for academic and pharmaceutical research environments focused on signal transduction, biomarker discovery, and pathway analysis.