Human SPR ELISA Kit (HUFI06346)

SPR (Sepiapterin Reductase) is the terminal NADPH-dependent enzyme in the tetrahydrobiopterin (BH4) biosynthetic pathway, where it reduces sepiapterin to yield 7,8-dihydrobiopterin. BH4 functions as an essential cofactor for phenylalanine hydroxylase, tyrosine hydroxylase, tryptophan hydroxylase, and the nitric oxide synthase family, which links SPR activity to monoaminergic neurotransmission, phenylalanine catabolism, and vascular nitric oxide signaling.

This quantitative immunoassay from Assay Genie is configured to measure human Sepiapterin Reductase in serum or plasma-derived specimens, supporting laboratory investigations into BH4 pathway regulation, hyperphenylalaninemia mechanisms, and disorders involving cofactor deficiency. Researchers studying DOPA-responsive dystonia, atypical phenylketonuria, endothelial nitric oxide signaling, and inflammatory conditions will find SPR quantification useful as a biomarker of cofactor biosynthetic capacity.

Each kit contains a pre-coated microplate, recombinant human SPR reference standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, chromogenic substrate solution, stop reagent, and adhesive plate seals. The assay is supplied in a multi-well plate format with sufficient reagents for 96 individual determinations and is intended for research use only. Store unopened reagents refrigerated at 4°C and allow the kit to equilibrate to room temperature before initiating the protocol.

For research use only.