Human SPINT1/HAI-1 ELISA Kit (HUFI06356)

SPINT1 (serine peptidase inhibitor, Kunitz type 1), also called HAI-1, is a type I transmembrane serine protease inhibitor that restrains matriptase, hepsin, and prostasin to maintain epithelial barrier integrity. Assay Genie supplies a quantitative immunoassay kit that measures human SPINT1/HAI-1 protein in research samples, supporting studies of the hepatocyte growth factor (HGF/MET) axis, placental biology, and tumor microenvironment biology in breast, colorectal, and gastric cancers where diminished HAI-1 expression correlates with invasion and metastasis.

Each kit ships with a pre-coated microplate strip plate, biotinylated detection antibody, recombinant protein standards, streptavidin-HRP conjugate, and wash buffer needed to quantify the inhibitor in cell culture supernatant and tissue lysate. Researchers apply the assay to characterize protease-inhibitor balance in wound healing, liver regeneration, and skin barrier models. Refrigerate components on arrival, aliquot working dilutions to limit freeze-thaw cycles, and protect the HRP conjugate from light. For research use only.