Human SPI1 ELISA Kit (HUFI06371)

Detect PU.1 (SPI1) transcription factor levels in human biological specimens with this colorimetric immunoassay from Assay Genie. SPI1 encodes the PU.1 protein, a member of the ETS family that governs hematopoietic lineage commitment and is studied in contexts including leukemia research, immune cell differentiation, and transcriptional regulation of lymphoid and myeloid progenitors.

The assay is supplied as a complete kit containing pre-coated microplate wells, detection antibodies, buffers, and a recombinant standard sufficient for 96 individual determinations. Researchers apply this kit to quantify SPI1 expression changes across cell culture experiments, patient-derived samples, and disease model systems where dysregulation of PU.1 contributes to oncogenic transformation or impaired hematopoiesis.

Store unopened components refrigerated between uses, protect the standard from light, and bring all reagents to room temperature before initiating the protocol. The workflow is intended for research use only and is not validated for diagnostic or clinical applications. Each kit supports a standard curve-based quantification approach compatible with downstream analyses of gene expression dynamics in immune and hematopoietic contexts.