Human SPHK2 ELISA Kit (HUFI06369)

Sphingosine kinase 2 (SPHK2), the enzyme that phosphorylates sphingosine to generate sphingosine 1-phosphate, can be quantified using the Assay Genie ELISA Kit. Researchers studying sphingolipid signaling in inflammation, cancer cell survival, or vascular biology use this plate-based assay to measure SPHK2 protein levels in prepared human serum or plasma fractions.

Each kit ships with a pre-coated microplate and provides all reagents needed to run the assay to completion. Detection relies on a chromogenic substrate read at an absorbance wavelength on a standard plate reader, and results are reported in standard-curve-derived concentration units. The product is supplied as a pack of reagents sufficient for 96 individual determinations in singlicate or 48 determinations in duplicate.

For storage and handling, unopened kits are kept refrigerated until first use; reagents should be brought to room temperature before running the assay to avoid condensation on plate wells. Researchers generally aliquot working-strength detection antibody to limit freeze-thaw cycles of the stock vial.