Human SPHK1-Phospho-Ser225 ELISA Kit (HUFI06367)

Phosphorylation of sphingosine kinase 1 at Ser225 is a key regulatory event in sphingolipid signaling, and quantifying this post-translational modification supports research into inflammation, vascular biology and tumor progression. This Assay Genie kit provides a colorimetric immunoassay workflow for measuring phospho-Ser225 SPHK1 in human specimens, formatted as a microplate assay with reagents sufficient for 96 determinations. Each kit is intended for research use only, supports cell culture and tissue lysate workflows alongside human serum or plasma specimens, and pairs a phospho-specific capture antibody with a conjugated detection antibody for absorbance readout. Refrigerated storage of the unopened kit preserves antibody binding activity through the printed shelf life, and the assay accommodates standard curve generation across multiple dilution points for accurate interpolation of unknowns.