Human Sphingosine kinase 2 (SPHK2) ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the quantitative detection of human sphingosine kinase 2 (SPHK2), also referred to as SK 2 or SK2. The assay uses colorimetric detection and is intended for research use only, providing a tool for investigating sphingolipid metabolism and its role in health and disease.

SPHK2 is a key enzyme that catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (SPP), a lipid mediator involved in cell signalling, growth, migration, and survival. Dysregulation of SPHK2 has been linked to various diseases including cancer, inflammation, and autoimmune disorders, making it a valuable target for therapeutic research. The kit reacts with human samples and has been validated for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant human SPHK2 (UniProt Q9NRA0).

According to the manufacturer datasheet, the kit provides a detection range of 15.6–1000 U/mL and a sensitivity of 7.8 U/mL. It is supplied for use in cardiovascular research, among other applications, and intra-assay CV, inter-assay CV, linearity, and recovery values are provided with the kit.