Human Spermidine/Spermine N1-Acetyltransferase 1 (SAT1) ELISA Kit

SAT1 (Spermidine/Spermine N1-Acetyltransferase 1) is the rate-limiting enzyme that catalyzes the acetyl-CoA-dependent acetylation of spermidine and spermine, positioning it as a central regulator of polyamine catabolism in human cells. The Assay Genie Human SAT1 ELISA Kit delivers a quantitative immunoassay for measuring SAT1 protein in tissue homogenates and cell lysates, supporting research into polyamine flux, cellular stress responses, and tumor cell biology.

Investigators studying responses to polyamine analogues, oxidative challenge, or cytotoxic chemotherapy rely on SAT1 quantification to track enzyme induction, since SAT1 upregulation is a recognized marker of active polyamine catabolism. The kit uses a matched antibody pair arranged in a microplate layout and yields an absorbance-based readout compatible with standard benchtop plate readers. Each kit is sized for 96 determinations and is configured for routine laboratory workflows.

The assay tolerates matrix variability typically encountered in homogenized tissue and whole-cell lysate preparations, allowing direct comparison across experimental conditions. Intended for research use only, this kit serves laboratories working on cell-cycle regulation, programmed cell death pathways, and the metabolic rewiring that accompanies proliferative disease states.