Human Sperm protamine P1/HSP1 ELISA Kit

This colorimetric Sandwich ELISA from Assay Genie is configured in a double-antibody format for the quantitative measurement of Sperm protamine P1 (HSP1) in human samples, with reactivity validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid sample types. The assay reports a detection range of 3.125–200 pg/mL and a sensitivity of 1.87 pg/mL, and is positioned for research applications in developmental biology.

Manufacturer performance data include linearity across 1:2, 1:4, and 1:8 dilutions: serum 80–103%, EDTA plasma 80–101%, and heparin plasma 81–100% (n=5). Mean recovery is 96% for serum (range 89–105%), 97% for EDTA plasma (range 90–104%), and 93% for heparin plasma (range 86–103%). The protocol uses sequential incubations with a biotinylated detection antibody and HRP-streptavidin conjugate, TMB substrate development, and absorbance reading at 450 nm. Kit reagents are stored at 2–8°C for up to 12 months.

The target analyte, also referred to as PRM1 and Cysteine-rich protamine (UniProt P04553), is found in the nucleus of sperm cells where it helps condense and stabilize DNA. According to the manufacturer's product description, abnormal protamine P1 levels have been associated with male infertility and sperm abnormalities, supporting its value as a biomarker in reproductive health research.