Human SPAG6 ELISA Kit (HUFI06377)

The Human SPAG6 ELISA Kit from Assay Genie is a sandwich-format immunoassay developed for quantitative measurement of Sperm-Associated Antigen 6 in human serum or plasma specimens. SPAG6 is a microtubule-binding axonemal protein originally characterized through its essential role in sperm flagellar assembly and ciliary motility, and it has since emerged as a candidate biomarker in reproductive biology and oncology research, including studies of acute myeloid leukemia and non-small cell lung carcinoma.

Each kit provides a complete set of reagents for an in vitro research workflow: pre-coated microplate wells, biotinylated detection antibody, recombinant human SPAG6 standard, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop solution, all packaged for a 96 Assays run. Researchers pipette diluted samples, incubate per the supplied protocol, and read absorbance to interpolate SPAG6 concentrations against the standard curve. The capture antibody is human-specific, with no significant cross-reactivity observed against related axonemal proteins in validation testing.

For best results, store kit components refrigerated between uses, protect the substrate from light, and avoid repeated freeze-thaw cycles of reconstituted standards. Reconstituted reagents should be aliquoted before storage. This product is intended for research use only and is not approved for diagnostic or clinical applications.