Human SPACA1 ELISA Kit (HUFI06387)

SPACA1, a transmembrane glycoprotein localized to the acrosomal cap of maturing spermatids, plays a role in sperm-egg membrane fusion during fertilization. This ELISA Kit from Assay Genie enables quantitative measurement of the protein in human research samples, supporting studies of spermatogenesis and male factor infertility. Homozygous loss-of-function variants in the SPACA1 gene have been linked to globozoospermia, a phenotype defined by round-headed, acrosome-less spermatozoa and severe reproductive failure.

The kit is supplied in a 96 Assays configuration and contains pre-titrated capture and detection antibodies, recombinant standards, and assay buffer needed to run the immunoassay workflow. Plates, diluents, and substrate reagents are organized for direct use in laboratories investigating acrosome biogenesis, idiopathic infertility cohorts, or candidate-gene follow-up after whole-exome sequencing.

Typical research applications include characterization of testicular biopsy material, profiling of infertile patient cohorts for acrosomal dysgenesis markers, and mechanistic studies of how SPACA1 expression changes during sperm maturation and capacitation. Reagents should be equilibrated and stored according to the package insert to preserve antibody binding activity and standard curve reproducibility across runs.