Human SP1 (Transcription factor Sp1) ELISA Kit (HUFI04863)

A Sandwich ELISA from Assay Genie designed for the quantitative detection of human Transcription factor Sp1 (SP1). The assay is colorimetric, with absorbance read at 450 nm, and is validated for use with serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. SP1 is a C2H2-type zinc-finger transcription factor that regulates gene expression, cell growth and differentiation, making it a relevant biomarker for research into cancer, cardiovascular and inflammatory disorders.

The reported sensitivity is 9.375 pg/mL with a standard range of 15.625-1000 pg/mL. The kit should be kept at 2-8°C for 12 months. The protocol uses a biotin-labeled detection antibody followed by HRP-Streptavidin conjugate and TMB substrate for color development, with absorbance measured at 450 nm after addition of stop solution. Known synonyms for the target include SP1, Sp1 transcription factor, Transcription factor Sp1 and TSFP1 (UniProt P08047).

Linearity across 1:2, 1:4 and 1:8 dilutions is 89-101%, 93-105% and 90-102% for serum (n=5), 88-99%, 88-105% and 84-100% for EDTA plasma (n=5), and 89-101%, 86-93% and 82-95% for heparin plasma (n=5). Reported recovery ranges and averages are 85-101% (92%) for serum, 87-103% (97%) for EDTA plasma and 86-101% (92%) for heparin plasma.