Human SOX5 ELISA Kit (HUFI06394)

The Assay Genie Human SOX5 ELISA Kit is built around a microplate immunoassay principle for quantifying SOX5 (SRY-Box Transcription Factor 5), a member of the SOX family that plays documented roles in chondrogenesis, cartilage formation, and has been investigated in several cancer pathways. The capture and detection antibodies used in this kit are specific to human SOX5 with minimal cross-reactivity to other SOX family members such as SOX6, SOX9, or L-SOX5, supporting accurate quantification in human-derived research samples. Researchers studying skeletal development, stem cell differentiation, or oncology biomarkers can use the kit to measure analyte levels in cell lysates and tissue homogenates prepared from human samples. The assay workflow involves adding samples and standards to the coated wells, incubating, washing, and adding detection reagents before reading absorbance on a standard plate reader. Standards are supplied as a lyophilized or liquid preparation for generating the standard curve, with dilution buffer provided in the kit. Reagent handling recommendations include aliquoting the standard to minimize repeated freeze-thaw cycles and protecting the chromogenic substrate from light. The lot-specific certificate of analysis supplied with the kit lists the exact sensitivity and standard curve range for that production lot, and storage instructions on the package insert should be followed. The kit is intended for research use only, not for diagnostic procedures.