Human SOX2 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise measurement of SOX2 (Sex Determining Region Y Box Protein 2) in human samples, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. SOX2 is a key transcription factor that plays a vital role in maintaining stem cell pluripotency and regulating cell differentiation, and is implicated in diseases such as cancer and neurodevelopmental disorders. The kit is therefore well suited to research in stem cell biology, developmental biology, and disease mechanisms, within the Epigenetics and Nuclear Signaling research area.

The assay uses a double-antibody sandwich format with colorimetric detection via TMB substrate, with absorbance read at 450 nm. The kit should be stored at 2-8°C for 12 months. Reported linearity across 1:2, 1:4 and 1:8 dilutions is 86-105%, 89-101% and 86-105% for serum (n=5), 89-99%, 91-97% and 84-95% for EDTA plasma (n=5), and 83-101%, 85-100% and 82-97% for heparin plasma (n=5). Spike-recovery averages are 96% for serum, 98% for EDTA plasma and 93% for heparin plasma.

Components supplied include a dismountable pre-coated ELISA microplate, lyophilized standard, biotin-labeled detection antibody (100X), HRP-Streptavidin conjugate (SABC, 100X), TMB substrate, sample dilution buffer, antibody dilution buffer, SABC dilution buffer, stop solution, wash buffer, plate sealers and a technical manual. The sample protocol comprises reagent and plate preparation, primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-Streptavidin binding for 30 minutes, and TMB color development in the dark for 10-20 minutes.