Human SOX12 ELISA Kit (HUFI06404)

SOX12 is a member of the SRY-related HMG-box family of transcription factors that influences chromatin remodeling and lineage specification during development, and its dysregulation has been studied in hepatocellular carcinoma, breast cancer, and other malignancies. This quantitative immunoassay from Assay Genie provides a microplate-based workflow for measuring human SOX12 protein levels in laboratory research settings.

Configured as a complete reagent set for in vitro research use only, the kit includes pre-coated plate wells, calibrated reference standards, a biotinylated detection reagent, and the wash, dilution, and substrate buffers needed to perform the assay. After sample addition and incubation, absorbance is recorded on a standard plate reader and SOX12 concentrations are interpolated from a standard curve. Reconstituted standards and concentrated reagents should be stored refrigerated at 4 degrees C and protected from light; avoid unnecessary freeze-thaw cycles to preserve antibody binding activity. The kit is supplied in a single-use, 96-assay microplate configuration suitable for processing multiple human research samples per run.

Typical research applications include profiling SOX12 expression in disease models, monitoring changes in cellular transcription factor levels following experimental treatments, and screening compound libraries for modulators of SOX12-regulated pathways. The assay is intended for research use only and is not validated for diagnostic procedures.