Human SOST PharmaGenie ELISA Kit (SBRS1111)

In bone metabolism research, accurate quantification of human sclerostin supports studies of osteocyte function, Wnt/β-catenin pathway activity, and disease states such as osteoporosis, sclerosteosis, van Buchem disease, and chronic kidney disease–mineral bone disorder. This in vitro, research-use-only ELISA from Assay Genie measures SOST in cell culture supernatants, plasma, and serum samples, providing a quantitative readout that pairs well with bone-turnover markers such as osteocalcin, P1NP, alkaline phosphatase, and CTX-1. Each kit ships with a pre-coated microplate, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer, substrate, and stop solution in individually sealed vials, with sufficient reagents for one full plate of measurements. The absorbance-based readout is captured on a standard plate reader, and concentrations are interpolated from a freshly prepared standard curve. Unopened kits should be refrigerated upon receipt and equilibrated to room temperature before use, while reconstituted standards and working dilutions are best aliquoted and stored to limit freeze-thaw cycles. The assay is intended for use with human samples only and is not cleared for clinical diagnostic procedures. Because sclerostin expression is dynamically regulated by mechanical loading, parathyroid hormone, glucocorticoids, and anti-sclerostin antibody therapies, the kit fits cleanly into time-course studies of bone-anabolic treatments, microgravity analogs, and fracture-healing models where osteocyte signaling is the variable of interest.