Human SORBS1 ELISA Kit

Manufactured by Assay Genie, this sandwich ELISA, double antibody kit enables quantitative measurement of SORBS1 in human serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay provides a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL. Standards and samples are incubated on the pre-coated plate, followed by binding of a biotin-labelled detection antibody, HRP-streptavidin incubation and color development with TMB substrate, with absorbance measured at 450 nm.

Linearity at 1:2, 1:4 and 1:8 dilutions falls within 96–105%, 87–101% and 85–101% for serum (n = 5), 82–97%, 82–98% and 86–99% for EDTA plasma (n = 5), and 82–99%, 81–100% and 85–95% for heparin plasma (n = 5). Recovery across the same matrices averages 95% in serum (range 90–101%), 94% in EDTA plasma (range 87–100%) and 94% in heparin plasma (range 85–103%). Unopened reagents are stored at 2–8°C for 12 months.

SORBS1, also referred to as Sorbin and SH3 domain-containing protein 1, c-Cbl-associated protein (CAP), SH3P12, Ponsin and SH3 domain protein 5 (UniProt Q9BX66), is implicated in signal transduction and in cellular processes including adhesion, migration and cytoskeletal organisation, with research interest in cancer, diabetes and cardiovascular disease contexts.