Human Sorbitol Dehydrogenase (SDH) ELISA Kit

Quantitative measurement of sorbitol dehydrogenase activity in human-derived samples is supported by this Assay Genie ELISA kit, which serves research applications in metabolic disorders, hepatic injury studies, and polyol pathway investigations. Sorbitol dehydrogenase is a zinc-containing cytoplasmic enzyme that catalyzes the NAD+-dependent interconversion of sorbitol and fructose, a metabolic step with relevance to diabetic complications and oxidative stress research.

The kit accommodates tissue homogenates alongside other human specimen types, supporting laboratory workflows focused on carbohydrate metabolism and enzyme biomarker quantification. Configured for 96 assays, it includes pre-coated microplate strips, calibrated reference standards, detection reagents, and a complete standard curve for accurate interpolation of unknown sample concentrations.

Application notes for this immunoassay include measuring hepatic enzyme leakage into circulation as a marker of cellular damage, profiling SDH expression in human tissue specimens, and supporting studies on alcoholic liver disease and polyol-related metabolic disturbances. The procedure follows standard ELISA methodology, with absorbance readings used to quantify enzyme levels across the calibration range.

Before initiating the assay, bring all reagents to room temperature and prepare serial dilutions of the reference standard. Running samples in duplicate or triplicate is recommended to reduce intra-assay variability and ensure reproducible optical density measurements across the plate.