Human Somatoliberin/GHRH ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the precise and reliable measurement of somatoliberin (also known as growth hormone-releasing hormone, GHRH) levels in human samples. The kit supports research into somatoliberin's role as a key regulator of the growth hormone pathway, with implications for physiological processes including growth, metabolism, and development. Dysregulation of somatoliberin has been implicated in disorders such as growth hormone deficiencies, acromegaly, and pituitary tumors, making it an important biomarker for these conditions.

The assay employs a colorimetric detection method with a reported sensitivity of 0.75 ng/mL and a standard range of 1.25–80 ng/mL. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, with storage at 2–8°C for 12 months. Recovery in serum averages 94%, EDTA plasma 92%, and heparin plasma 96%. Linearity across 1:2, 1:4, and 1:8 dilutions ranges from 82% to 105% across serum, EDTA plasma, and heparin plasma. The procedure uses a pre-coated plate with primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-Streptavidin (SABC) binding, TMB substrate development in the dark, and absorbance measurement at 450 nm.