Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit

Researchers studying intracellular pH regulation, cardiac ischemia-reperfusion injury, and tumor cell migration frequently need a quantitative readout of the sodium/hydrogen exchanger 1 (NHE1) antiporter, the ubiquitous SLC9A1-encoded plasma membrane transporter that exchanges one intracellular proton for one extracellular sodium ion. NHE1 governs cell volume control, cytoskeletal organization, focal adhesion turnover, and the early phase of apoptotic cell shrinkage, and its dysregulation is implicated in heart failure, stroke, and cancer invasion.

This ELISA from Assay Genie is configured for measurement of human NHE1 protein in tissue homogenates and cell lysates, providing a 96-assay plate layout for research use only. The kit enables laboratories studying cancer cell invasion, myocardial pH regulation, and intracellular acid-base homeostasis to obtain protein-level data that complements transcript and activity measurements. Unopened kits and working reagents should be kept refrigerated at 4 °C; prepared samples are best aliquoted and frozen to limit freeze-thaw cycles. Each pack supplies a pre-coated microplate, lyophilized standards, detection antibody, substrate, and the buffers needed to run the full plate, with a protocol detailing sample preparation, incubation times, and data analysis included.