Human Sodium/glucose cotransporter 2 (SLC5A2) ELISA Kit

A Sandwich ELISA Kit from Assay Genie designed for the accurate detection of SLC5A2 levels in human serum, plasma, and cell culture supernatants. The kit provides high sensitivity and specificity for precise and consistent results. SLC5A2 is a key protein involved in the regulation of glucose reabsorption in the kidney, playing a crucial role in glucose homeostasis; its dysregulation has been implicated in conditions such as diabetes and kidney disorders, making it a valuable biomarker for research.

The assay is reactive with human samples and is suitable for serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. It detects natural and recombinant human Sodium/glucose cotransporter 2, also known as SGLT2, low affinity sodium-glucose cotransporter, solute carrier family 5 member 2, and Na(+)/glucose cotransporter 2. The reported detection range is 0.156–10 ng/mL with a sensitivity of 0.089 ng/mL. Intra-assay CV is 6.4% and inter-assay CV is 10.0%. Recovery ranges from 97–109% (average 103%) for serum and 99–111% (average 105%) for plasma. Linearity has been validated across serum, EDTA plasma, and heparin plasma at dilution factors from 1:2 to 1:16.

The target is a multi-pass membrane protein (UniProt P31639) located on the plasma membrane. In the kidney, efficient substrate transport is provided by the concerted action of a low affinity high capacity and a high affinity low capacity Na(+)/glucose cotransporter arranged in series along kidney proximal tubules. The kit is intended for research use only.