Human SNX5 ELISA Kit (HUFI06419)

This kit from Assay Genie enables quantitative detection of SNX5 (Sorting Nexin 5), a peripheral membrane protein of the sorting nexin family that drives endosomal trafficking and retromer-mediated cargo retrieval. SNX5 contains a PX domain binding phosphatidylinositol 3-phosphate and forms heterodimers with SNX1 or SNX2 to regulate receptor recycling, including EGFR and Wnt pathway components, making this assay useful for cancer research, receptor biology, and investigations of intracellular membrane transport.

The protocol uses a 50 µL sample volume per well with all reagents supplied ready to use. Components remain stable when stored at 4°C; the standard and antibody vials should be kept at -20°C for long-term storage. Each pack contains a pre-coated microplate, biotinylated detection antibody, Streptavidin-HRP conjugate, dilution buffer, wash buffer, and TMB substrate sufficient for the full plate run, supporting applications in cell signaling, oncology, and membrane trafficking research.