Human SNTN (Sentan) ELISA KIT (HUFI04887)

Built by Assay Genie, this quantitative immunoassay detects human Sentan (SNTN), a flagellar-tip protein implicated in sperm motility and final spermatid maturation in humans. The kit arrives as a complete package containing a pre-coated microplate, lyophilized standards, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, and TMB substrate. Researchers in reproductive biology, andrology, and ciliopathy studies such as primary ciliary dyskinesia commonly use the kit for in vitro analyte profiling from biological matrices including cell lysates and tissue homogenates. Reconstituted standards should be aliquoted and stored frozen at -80°C to prevent repeated freeze-thaw cycles, while unused microplate strips can be refrigerated in the sealed pouch. The protocol produces absorbance readings on a standard plate reader, with results plotted against a multi-point standard curve. Each kit provides reagents for 96 individual determinations, and the procedure can be completed in approximately 4.5 hours. The kit is intended for research use only and is not validated for clinical diagnostic applications.