Human SNRPF (Small nuclear ribonucleoprotein F) ELISA Kit (HUFI06414)

SNRPF encodes the F subunit of the heptameric Sm ring that caps the U1, U2, U4/U6, and U5 small nuclear ribonucleoproteins in the spliceosome. This Assay Genie immunoassay supports quantitative measurement of human SNRPF, with applications in spliceosome assembly research and Sm-protein autoimmunity; anti-Sm autoantibodies characteristic of systemic lupus erythematosus (SLE) target this ring complex.

The microplate is pre-coated with a human-specific capture antibody; standards, samples, and a biotinylated detection antibody are added to wells, followed by streptavidin-HRP and a chromogenic substrate read at 450 nm. Each vial of reconstituted standard supports an eight-point standard curve, and the protocol calls for a 20 µL sample volume per well. SNRPF is a low-molecular-weight protein of approximately 9.7 kDa, and users studying cell or tissue lysates may pair this ELISA kit with an anti-SNRPF antibody for orthogonal validation by western blot.