Human Snf1lk2/SIK2 ELISA Kit

A competitive ELISA kit from Assay Genie is designed for the quantitative measurement of human SNF1LK2, also known as SIK2, in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay uses a colorimetric, competitive format in which the plate is coated with antigen and target is quantified using a biotin-labeled antibody, HRP-streptavidin conjugate, and TMB substrate, with absorbance read at 450 nm. The target is referenced under UniProt Q9H0K1 and falls within the Cell Biology research area.

SNF1LK2/SiK2 is described as a key protein involved in cellular processes such as metabolism, energy homeostasis, and gene expression regulation, and its dysregulation has been linked to diabetes, obesity, and cancer, making it a useful biomarker for research in these conditions. The kit reports a sensitivity of 0.188 ng/mL and an assay range of 0.313–20 ng/mL.

Reported linearity values across dilutions 1:2, 1:4, and 1:8 are 85–103%, 89–97%, and 91–99% for serum (n = 10); 82–101%, 89–101%, and 84–94% for EDTA plasma (n = 10); and 84–96%, 85–100%, and 82–98% for heparin plasma (n = 10). Mean recovery is 96% in serum, 94% in EDTA plasma, and 95% in heparin plasma. Kits should be stored sealed at 2–8°C and must not be cryopreserved.