Human SMURF2 (E3 ubiquitin-protein ligase SMURF2) ELISA Kit (HUFI04879)

Assay Genie supplies this in vitro research-use quantitative immunoassay for measuring E3 ubiquitin-protein ligase SMURF2 in human specimens. SMURF2 (SMAD Ubiquitination Regulatory Factor 2) is a HECT-domain E3 ubiquitin ligase that regulates SMAD transcription factors and downstream TGF-beta signaling, with documented roles in cancer cell proliferation, DNA damage response, and autophagy. Investigators studying the ubiquitin-proteasome pathway, receptor turnover, and signal transduction can apply this kit to track endogenous SMURF2 protein levels across experimental conditions.

The kit ships with pre-coated microplate strips, lyophilized recombinant standards, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, wash concentrate, and stop solution — enough reagents for 96 individual determinations per plate. The protocol accommodates cell lysate and tissue homogenate alongside human biofluid samples, supporting both cell-based investigations and translational research workflows. Each microplate well provides a uniform capture surface for the target analyte, and the workflow proceeds through sequential incubation steps culminating in an absorbance readout on a standard plate reader.

For reagent stability, keep unopened kit components refrigerated at 4°C and protect the TMB substrate from light. Reconstituted standards and any diluted samples should be kept refrigerated and used within the working day when practical; avoid repeated freeze-thaw cycles of working dilutions. The kit is intended for research use only and has not been validated for diagnostic decision-making. Run a full standard curve on every plate, include appropriate positive and negative controls, and refer to the lot-specific certificate of analysis provided by Assay Genie with each shipment.