Human SMO ELISA Kit (HUFI06436)

The Assay Genie Human Smoothened (SMO) ELISA Kit enables quantitative measurement of the Smoothened transmembrane protein, the signal-transducing component of the Hedgehog pathway cascade, in research specimens using pre-titrated capture and detection antibodies on a pre-coated microplate. The kit is validated specifically for the human Smoothened target, with all calibrators and detection reagents raised against the recombinant human isoform. SMO biology is centrally relevant to oncology and developmental patterning investigations: activating mutations in SMO drive basal cell carcinoma and medulloblastoma, while pharmacological SMO inhibitors such as vismodegib and sonidegib are reference tools in Hedgehog-pathway drug screening studies.

This 96 Assays format kit is supplied with pre-titrated capture antibody, biotinylated detection reagent, lyophilized recombinant human Smoothened standards, streptavidin-HRP conjugate, washing buffer, and TMB chromogenic substrate. The microplate is configured in removable eight-well strips, allowing smaller experiments without wasting reagents, and full-plate workflows are supported for larger screening campaigns. The recommended protocol dispenses 100 µL of sample per well.

Unopened kit components should be stored refrigerated at 4 degrees C until the printed expiration date. Reconstituted standards and diluted detection antibody should be aliquoted into single-use vials and frozen at -20 degrees C or lower to limit repeated freeze-thaw cycles. This product is supplied for research use only and is not cleared for diagnostic applications.