Human SMAD4 PharmaGenie ELISA Kit (SBRS1103)

SMAD4 (Mothers against decapentaplegic homolog 4) is a key mediator of TGF-beta signal transduction, and this Assay Genie ELISA kit enables quantitative measurement of the protein in research samples. SMAD4 functions as a tumor suppressor and is frequently inactivated in pancreatic ductal adenocarcinoma, colorectal cancer, and juvenile polyposis syndrome, where homozygous deletion or loss of heterozygosity at chromosome 18q21.1 contributes to disease progression. Scientists studying TGF-beta pathway dysregulation, epithelial-mesenchymal transition, or cancer biology apply this kit to quantify SMAD4 protein levels in cell culture supernatants. The immunoassay supports investigations into BMP and activin signaling cascades, where SMAD4 forms heteromeric complexes with receptor-activated SMADs (R-SMADs) to regulate transcription of target genes such as p21 and PAI-1. Each kit provides reagents to perform 96 determinations using a microplate-based format intended for research use only.