Human SMAD2 ELISA Kit

From Assay Genie, this Sandwich ELISA Kit is designed for the precise detection of SMAD2 levels in human samples, including serum, plasma, and cell culture supernatants. SMAD2 is a key mediator of the TGF-beta signaling pathway, contributing to cell differentiation, proliferation, and apoptosis. Dysregulation of SMAD2 has been linked to cancer, fibrosis, and developmental disorders, making it a useful biomarker for studying these conditions.

The double antibody sandwich format uses colorimetric detection, with a stated sensitivity of 0.188 ng/mL and a range of 0.313-20 ng/mL. Acceptable sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is stored at 2-8°C for 12 months and is classified under the Epigenetics and Nuclear Signaling research area, with Uniprot reference Q15796.

Reported linearity values across 1:2, 1:4, and 1:8 dilutions are 91-102%, 86-100%, and 82-98% for serum (n=5); 86-103%, 82-91%, and 81-100% for EDTA plasma (n=5); and 92-101%, 84-89%, and 82-99% for heparin plasma (n=5). Average recovery values are 95% for serum (range 89-105%), 92% for EDTA plasma (range 85-100%), and 96% for heparin plasma (range 89-103%). The procedure involves reagent preparation, a 90-minute primary incubation at 37°C, a 60-minute detection antibody incubation at 37°C, a 30-minute HRP-streptavidin step at 37°C, 10-20 minutes of TMB substrate color development in the dark, and absorbance reading at 450 nm after stop solution is added.