Human Smad1/MADH1 ELISA Kit

Assay Genie offers a Sandwich ELISA, Double Antibody kit for the quantitative detection of Smad1/MADH1 in human samples. The assay is designed to measure SMAD1 levels in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is intended for research within the Epigenetics and Nuclear Signaling area.

The assay has a sensitivity of 0.469 ng/mL and a range of 0.781-50 ng/mL. Reported linearity values for 1:2, 1:4 and 1:8 dilutions are 86-102%, 85-99% and 80-100% for serum (n = 5), 86-104%, 82-101% and 88-99% for EDTA plasma (n = 5), and 90-103%, 87-99% and 87-99% for heparin plasma (n = 5). Reported recovery ranges and averages are 90-96% (average 93%) for serum (n = 5), 86-101% (average 94%) for EDTA plasma (n = 5) and 85-103% (average 96%) for heparin plasma (n = 5).

The procedure involves a primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody incubation at 37°C for 60 minutes, HRP-Streptavidin (SABC) incubation at 37°C for 30 minutes, TMB color development in the dark for 10-20 minutes, and a stop reaction with absorbance measured at 450 nm. SMAD1, also known as MADH1, is described as a key player in the TGF-beta signaling pathway, regulating cell growth, differentiation and apoptosis, with dysregulation implicated in cancer, developmental disorders and bone homeostasis abnormalities.