Human SLU7 ELISA Kit (HUFI06445)

The Assay Genie Human SLU7 ELISA Kit enables quantitative detection of SLU7 protein, a zinc-knuckle splicing factor essential for the second catalytic step of pre-mRNA splicing and accurate 3' splice site selection. Investigators working on hepatocellular carcinoma, alcoholic liver disease, hepatic mRNA processing defects, and tumor spliceosome dysregulation use this kit to evaluate SLU7 expression levels. Supplied as a 96 Assays pack, all critical components arrive in sealed vials including pre-coated microplate wells, lyophilized recombinant standard, biotinylated detection antibody, and substrate solution. Reconstituted reagents should be refrigerated and protected from light; aliquot standards to minimize repeated freeze-thaw cycles and preserve antibody binding activity. Each kit is intended for research use only and is validated specifically for human reactivity, making it a reliable tool for translational studies comparing SLU7 abundance across patient cohorts, disease stages, or treatment conditions. The protocol accommodates routine immunoassay workflows and integrates readily with orthogonal methods such as western blot, RNA-seq validation, and immunohistochemistry, supporting cross-platform confirmation of splicing-factor changes. Researchers can leverage the included standard curve to interpolate unknown concentrations, while detailed insert instructions cover reagent preparation, wash stringency, incubation times, and data analysis. Consistent inter- and intra-assay reproducibility allows direct comparison between biological replicates, time-course experiments, and independent sample batches. This makes the kit particularly valuable for labs exploring aberrant splice site usage in liver pathology, cancer biomarker discovery, and mechanistic dissection of the human spliceosome.