Human SLC7A4 ELISA Kit (HUFI06458)

Assay Genie's Human SLC7A4 ELISA Kit enables quantitative measurement of the cationic amino acid transporter SLC7A4 in human research specimens. SLC7A4 is a transmembrane glycoprotein within the solute carrier family 7, primarily involved in mediating the transport of cationic amino acids across cellular membranes. Research interest in SLC7A4 has expanded in recent years due to its emerging role in tumor metabolism, cancer cell proliferation, and the tumor immune microenvironment, with published studies examining its expression in prostate, breast, and colorectal carcinoma tissues. Investigators use this microplate-based immunoassay to quantify circulating SLC7A4 levels in translational oncology projects, biomarker validation studies, and amino acid transport pathway investigations. The plate is pre-coated with monoclonal capture antibody selective for human SLC7A4, paired with a biotinylated detection reagent and streptavidin-HRP conjugate to amplify signal output during the chromogenic development step. Reconstituted protein standards support generation of an eight-point calibration curve for accurate interpolation of unknown specimen concentrations. To preserve antibody activity, the kit should be stored refrigerated upon arrival, with all working dilutions aliquoted into single-use vials prior to freezing to prevent degradation from repeated freeze-thaw events.