Human SLC7A11/XCT ELISA Kit

Assay Genie provides this sandwich double-antibody ELISA for the quantitative measurement of human SLC7A11/XCT (also known as xCT, cystine/glutamate transporter, solute carrier family 7 member 11, amino acid transport system xc-, and CCBR1). The microplate is pre-coated with a capture antibody specific to the target; standards and diluted samples are added and incubated, a biotinylated detection antibody then forms a sandwich complex, and HRP-conjugated streptavidin with TMB substrate produces a colorimetric reaction whose absorbance is read at 450 nm.

Validated sample matrices include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Linearity across 1:2–1:8 dilutions is serum 84–104%, EDTA plasma 85–101%, and heparin plasma 80–99%. Recovery ranges are serum 86–105% (average 96%), EDTA plasma 93–100% (average 95%), and heparin plasma 91–105% (average 97%). The kit is stored at 2–8°C for 12 months and is intended for research use within the signal transduction area.

The procedure runs as follows: equilibrate reagents and TMB substrate to room temperature, load standards/samples/blanks and incubate the plate at 37°C for 90 minutes, add biotin-labeled detection antibody and incubate for 60 minutes, add HRP Streptavidin (SABC) and incubate for 30 minutes, develop with TMB in the dark for 10–20 minutes, then add stop solution and read absorbance at 450 nm immediately.