Human SLC6A8 ELISA Kit (HUFI06478)

SLC6A8 is the gene encoding the human creatine transporter (CRT), a plasma membrane protein responsible for cellular creatine uptake, and this research-use immunoassay from Assay Genie enables quantitative measurement of the transporter in serum-derived specimens and plasma-derived specimens. Mutations at the Xq28 locus cause X-linked creatine transporter deficiency, a neurodevelopmental and neurometabolic disorder associated with intellectual disability, seizures, delayed speech, and hypotonia, making the transporter a target of active biomarker research.

Each kit is configured for 96 individual determinations and ships with pre-coated microplate wells, lyophilized reference standards, biotinylated detection reagent, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop solution. The procedure yields a colorimetric read-out measured on a standard microplate absorbance instrument, and the included standards support construction of a multi-point standard curve. Reconstituted reagents and unused strips should be stored refrigerated to preserve activity until the next run.

Beyond classical deficiency studies, the kit is deployed in investigations of muscle creatine handling, neurological disease mechanisms, and pharmacological screening of compounds that modulate SLC6A8 transport activity. Detailed dilution recommendations, incubation times, and wash protocols are included in the product insert. For research use only; not for clinical diagnostic applications.