Human SLC16A8 ELISA Kit

Manufactured by Assay Genie, this Sandwich ELISA Kit is designed for the precise quantification of SLC16A8, also known as monocarboxylate transporter 3 (MCT3), in human biological samples. The kit uses a double-antibody sandwich ELISA format and is intended for research applications in signal transduction and metabolic studies, where SLC16A8 levels may be relevant to disorders such as diabetes, obesity, and cancer.

The kit has a stated sensitivity of 0.375 ng/mL and an assay range of 0.625-40 ng/mL. Acceptable sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Components should be stored at 2-8°C, where the kit remains stable for 12 months.

Validation data report linearity between 82-105% across serum, EDTA plasma, and heparin plasma at 1:2, 1:4, and 1:8 dilutions (n=5). Recovery ranges from 88-104% for serum (average 96%), 88-99% for EDTA plasma (average 95%), and 89-101% for heparin plasma (average 95%). The assay procedure involves a primary incubation at 37°C for 90 minutes, detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and absorbance reading at 450 nm after stop solution addition.