Human Sirtuin 2 (SIRT2) ELISA Kit

SIRT2 (Sirtuin 2) is an NAD+-dependent class III deacetylase that operates predominantly in the cytoplasm, where it deacetylates alpha-tubulin, histones, and metabolic enzymes to regulate cell cycle progression, mitotic checkpoint fidelity, and lipid homeostasis. Altered SIRT2 expression has been documented in human malignancies, in Parkinson's and Huntington's disease pathology, and in metabolic disturbances including insulin resistance and hepatic steatosis. This immunoassay kit from Assay Genie provides a quantitative tool to measure endogenous human SIRT2 protein levels, supporting cancer biology, neurobiology, and metabolic disease research.

The complete package contains a microplate pre-coated with capture antibody, biotinylated detection antibody, calibrated reference standards, streptavidin-HRP conjugate, substrate solution, and wash buffer, sufficient for 96 individual determinations. It accepts diverse human specimen types including serum samples, plasma specimens, tissue homogenates, whole-cell lysates, and conditioned cell culture media, enabling both cell-based studies and translational investigations. The assay yields a measurable signal compatible with routine laboratory plate readers and is intended for research use only.