Human SIRT3 (NAD-dependent ADP-ribosyltransferase sirtuin-4) ELISA Kit (HUFI03404)

The Assay Genie SIRT3 ELISA kit enables quantitative measurement of human Sirtuin-3, a mitochondrial NAD-dependent deacetylase that regulates oxidative phosphorylation, fatty acid oxidation, and reactive oxygen species homeostasis. Investigators working on metabolic syndrome, cardiac stress responses, neurodegeneration, and tumor bioenergetics rely on circulating SIRT3 levels as a readout of mitochondrial integrity in clinical and preclinical samples.

The 96 Assays format provides pre-coated microplate wells, detection antibody, recombinant standards, and substrate reagents sufficient to generate a full standard curve alongside experimental unknowns. Colorimetric readout integrates with standard benchtop plate readers, and the protocol accommodates tissue homogenate and cell lysate inputs collected from human donors and cultured cell lines.

For research use only. Store the unopened kit refrigerated and bring all components to room temperature before each run; reconstituted standards should be aliquoted to limit freeze-thaw cycles. The kit pairs naturally with downstream western blot confirmation and with cell culture experiments probing mitochondrial function under nutrient or oxidative challenge.