Human SIL1 ELISA Kit (HUFI06519)

Quantify human SIL1 protein with this enzyme-linked immunosorbent research kit from Assay Genie, supplied in a 96-assay microplate format intended for in vitro use. SIL1 is an endoplasmic reticulum-resident nucleotide exchange factor that restocks ATP on BiP/GRP78, the ER-resident Hsp70 chaperone, and drives nascent polypeptide folding within the ER lumen. Loss-of-function mutations in SIL1 cause Marinesco-Sjögren syndrome, an autosomal recessive cerebellar ataxia with myopathy and cataracts, making SIL1 a focus of proteostasis research, unfolded protein response studies, and IRE1-XBP1 pathway investigations. The kit accepts diverse human sample matrices, contains all buffers and reagents required to run a full microplate of determinations, and produces a colorimetric readout compatible with standard plate readers. Kit reagents are shipped refrigerated and should be stored refrigerated while protected from freeze-thaw cycles to preserve activity. Combine SIL1 measurements with BiP expression readouts or downstream ER stress markers such as spliced XBP1 to map chaperone cycling, heat shock response dynamics, and stress-related signaling in cell culture systems and in vivo model studies.